Peroxide-driven cytochrome P450 oxygenase variants
US7465567B2 · kind B2 · utility
10Cited by
17References
41Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Apr 16, 2002 |
| Grant date | Dec 16, 2008 |
| Priority date | — |
| Expiry date | Apr 16, 2022 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y114/14001
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention relates to novel variants of cytochrome P450 oxygenases. These variants have an improved ability to use peroxide as an oxygen donor as compared to the corresponding wild-type enzyme. These variants also have an improved thermostability as compared to the cytochrome P450 BM-3 F87A mutant. Preferred variants include cytochrome P450 BM-3 heme domain mutants having I58V, F87A, H100R, F107L, A135S, M145A/V, N239H, S274T, L324I, I366V, K434E, E442K, and/or V446I amino acid substitutions.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.