Catalytic domains of β(1,4)-galactosyltransferase I having altered donor and acceptor specificities, domains that promote in vitro protein folding, and methods for their use

US7482133B2 · kind B2 · utility

Assignee

• The United States of America, as represented by the Department of Health and Human Services · US

Inventors

• Pradman K. Qasba · Bethesda, US
• Boopathy Ramakrishnan · Braintree, US

Key dates

• Filing date: Jul 8, 2005
• Grant date: Jan 27, 2009
• Expiry date: Jan 27, 2026

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Y204/01133
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Disclosed are methods and compositions that can be used to synthesize oligosaccharides; mutants of galactosyltransferases having altered donor and acceptor specificity; methods for increasing the immunogenicity of an antigen; and polypeptide stem regions that can be used to promote in vitro folding of polypeptides, such as the catalytic domain from a galactosyltransferase.