Patent · US Expired

Method for the purification of an N-terminal fragment of hepatocyte growth factor

US7544493B2 · kind B2 · utility

0Cited by

2References

8Claims

0Family size

Assignee

Hoffmann-La Roche Inc. · US

Inventors

Friederike Hesse · Munich, DE
Martin Lanzendoerfer · Tutzing, DE
Apollon Papadimitriou · Bichl, DE
Jan Olaf Stracke · Penzberg, DE

Key dates

Filing date	Mar 2, 2005
Grant date	Jun 9, 2009
Priority date	—
Expiry date	Oct 21, 2025

Classification

Technology area (CPC A)Human Necessities
CPC primaryA61P43/00
WIPO fieldPharmaceuticals
WIPO sectorChemistry

Abstract

A method for the production of the N-terminal four kringle-containing fragment of hepatocyte growth factor (NK4) by expression of a nucleic acid encoding said NK4 in a microbial host cell, isolation of inclusion bodies containing said NK4 in denatured form, solubilization of the inclusion bodies and naturation of the denatured NK4, characterized in that solubilization and naturation are performed at pH 7-9 in phosphate buffered solution, provides NK4 in high purity and high yield.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.