Detection method using dissociated rolling circle amplification
US7553619B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Feb 8, 2002 |
| Grant date | Jun 30, 2009 |
| Priority date | — |
| Expiry date | Feb 8, 2022 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6804
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Disclosed are compositions and methods for detecting small quantities of analytes such as proteins and peptides. The method involves associating a DNA circle with the analyte and subsequent release and rolling circle replication of the circular DNA molecule. In the method, an amplification target circle is associated with analytes using a conjugate of the circle and a specific binding molecule that is specific for the analyte to be detected. Amplification target circles not associated with the proteins are removed, the amplification target circles that are associated with the proteins are decoupled from the specific binding molecule and amplified by rolling circle amplification. The amplification is isothermic and can result in the production of a large amount of nucleic acid from each primer. The amplified DNA serves as a readily detectable signal for the analytes.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.