Linear amplification of short nucleic acids
US7556943B2 · kind B2 · utility
2Cited by
2References
24Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | May 31, 2006 |
| Grant date | Jul 7, 2009 |
| Priority date | — |
| Expiry date | May 31, 2026 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6853
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present teachings provide novel methods for amplifying short nucleic acids. In some embodiments, the present teachings provide novel methods for linearly amplifying a collection of micro RNAs by using temperature cycling during a reverse transcription reaction. The cycling can comprise at least 20 cycles of an annealing temperature segment of 10 C-30 C, and a denaturation temperature segment of 35 C-60 C. In some embodiments, the temperature cycled reaction can comprise an osmolyte.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.