Methods, compositions, and kits for forming self-complementary polynucleotides
US7588896B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Jan 12, 2007 |
| Grant date | Sep 15, 2009 |
| Priority date | — |
| Expiry date | Feb 9, 2027 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6876
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present teachings generally relate to methods, kits, and compositions for detecting target polynucleotide sequences. The teachings also relate to ligation and amplification reactions that generate self-complementary polynucleotide products. In some embodiments, ligation reactions are performed with probes that result in the formation a self-complementary ligation product. Ligation of a hairpin linker to the self-complementary ligation product can form a loop ligation product. In some embodiments, the loop ligation product can be amplified with rolling circle amplification. Detection of a loop ligation product can serve to determine the identity of a target polynucleotide.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.