Polymerase enhancing factor (PEF) extracts, PEF protein complexes, isolated PEF proteins, and methods for purifying and identifying them
US7625725B1 · kind B1 · utility
1Cited by
37References
4Claims
0Family size
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Key dates
| Filing date | Aug 4, 2000 |
| Grant date | Dec 1, 2009 |
| Priority date | — |
| Expiry date | Jan 21, 2021 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/686
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention provides novel extracts, proteins, and complexes that improve the polymerization activity of nucleic acid polymerases. Included within the aspects of the invention are methods for identifying compositions with a polymerase enhancing activity, methods for purifying and using these compositions, and specific extracts, proteins, and complexes that function to enhance polymerase activity. As an example, specifically described is nucleotide and amino acid sequence information for Pyrococcus furiousus PEF (P45), which was used to produce a recombinant PEF.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.