Optical microscopy with phototransformable optical labels

US7626703B2 · kind B2 · utility

Inventors

• Robert Eric Betzig · Ashburn, US
• Harald F. Hess · Ravensburg, DE

Key dates

• Filing date: Nov 21, 2007
• Grant date: Dec 1, 2009
• Expiry date: Jan 12, 2028

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2021/6441
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Spatially-structured activation radiation having relatively high- and low-intensity regions is provided to a sample that includes phototransformable optical labels (“PTOLs”). A subset of the PTOLs located predominately at relatively high intensity regions of the spatially-structured activation radiation is activated. Spatially-structured excitation radiation is provided to the activated PTOLs, and the excitation radiation is structured so that one or more relatively high intensity regions of the excitation radiation at least partially overlap one or more relatively high intensity regions of the activating radiation to excite PTOLs located at the relatively high intensity excitation radiation regions. Radiation emitted from the activated and excited PTOLs is detected with imaging optics. Intensities and spatial structures of activating radiation and excitation radiation are controlled so radiation emitted from activated and excited PTOLs is emitted substantially from a volume that is comparable to or less than a diffraction-limited resolution volume (“DLRV”) of the imaging optics.