Gold implantation/deposition of biological samples for laser desorption two and three dimensional depth profiling of biological tissues
US7629576B2 · kind B2 · utility
18Cited by
17References
57Claims
0Family size
Assignee
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Key dates
| Filing date | Sep 21, 2005 |
| Grant date | Dec 8, 2009 |
| Priority date | — |
| Expiry date | Sep 21, 2025 |
Classification
- Technology area (CPC H)Electricity
- CPC primaryH01J49/0418
- WIPO fieldElectrical machinery, apparatus, energy
- WIPO sectorElectrical engineering
Abstract
The present invention enhances the laser desorption of biological molecular ions from surfaces by creating a surface localized MALDI particle matrix by ion implantation of low energy ionized clusters (gold, aluminum, etc.) or chemically derivatized clusters into the near surface region of the sample. MALDI analysis of the intact biomolecules on the surface or within a narrow subsurface region defined by the implantation range of the ions can then be performed by laser desorption into a mass spectrometer or, in a preferred embodiment, into a combined ion mobility orthogonal time of flight mass spectrometer.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.