Labeled peptides, proteins and antibodies and processes and intermediates useful for their preparation

US7662644B2 · kind B2 · utility

Assignee

• THE SCRIPPS RESEARCH INSTITUTE · US

Inventors

• Klaus Hahn · Chapel Hill, US
• Alexei Toutchkine · Chapel Hill, US
• Rajeev Muthyala · Champaign, US
• Vadim Kraynov · San Diego, US
• Steven Bark · San Diego, US
• Dennis R. Burton · Village of La Jolla, US
• Chester Chamberlain · Cambridge, US

Key dates

• Filing date: Feb 12, 2007
• Grant date: Feb 16, 2010
• Expiry date: Nov 7, 2027

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K1/1077
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The invention provides peptide synthons having protected functional groups for attachment of desired moieties (e.g. functional molecules or probes). Also provided are peptide conjugates prepared from such synthons, and synthon and conjugate preparation methods including procedures for identifying optimum probe attachment sites. Biosensors are provided having functional molecules that can locate and bind to specific biomolecules within living cells. Biosensors can detect chemical and physiological changes in those biomolecules as living cells are moving, metabolizing and reacting to its environment. Methods are included for detecting GTP activation of a Rho GTPase protein using polypeptide biosensors. When the biosensor binds GTP-activated Rho GTPase protein, an environmentally sensitive dye emits a signal of a different lifetime, intensity or wavelength than when not bound. New fluorophores whose fluorescence responds to environmental changes are also provided that have improved detection and attachment properties, and that can be used in living cells, or in vitro.