Animal model for the fast identification of pharmaceutical active compounds in vivo
US7666670B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Mar 3, 2004 |
| Grant date | Feb 23, 2010 |
| Priority date | — |
| Expiry date | Mar 3, 2024 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA01K2267/0393
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
In a first aspect the present invention provides a tumor cell line stably transfected with an expression vector containing a reporter gene, preferably a fluorescent protein, operably linked to a promotor that also controls expression of a protein that is associated with tumor regression, stabilization of tumor growth or inhibition of metastatic growth, characterized in that said cell line is capable to form a tumor when implanted or injected into the non-human animal. Compared to the traditional in vivo models, the present invention differs in that the reporter gene is not constitutively expressed, but only after exposure to a test compound that results in the expression of a protein or enzyme associated with tumor regression, stabilization of tumor growth or inhibition of metastatic growth. Only when a compound to be tested got into circulation and infiltrated the tumor it may generate the reporter signal, provided it promotes the expression of a protein associated with tumor regression and the promoter of said protein is operably linked to the reporter gene.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.