Robust expression of a bioactive mammalian protein in chlamydomonas chloroplast
US7678561B2 · kind B2 · utility
3Cited by
3References
51Claims
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Key dates
| Filing date | May 9, 2007 |
| Grant date | Mar 16, 2010 |
| Priority date | — |
| Expiry date | May 9, 2027 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/8257
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods and compositions are disclosed to engineer chloroplast comprising heterologous mammalian genes via a direct replacement of chloroplast Photosystem II (PSII) reaction center protein coding regions to achieve expression of recombinant protein above 5% of total protein. When algae is used, algal expressed protein is produced predominantly as a soluble protein where the functional activity of the peptide is intact. As the host algae is edible, production of biologics in this organism for oral delivery or proteins/peptides, especially gut active proteins, without purification is disclosed.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.