Patent · US Active

Optical microscopy with phototransformable optical labels

US7782457B2 · kind B2 · utility

30Cited by

19References

88Claims

0Family size

Assignee

Hestzig LLC · US

Inventors

Robert Eric Betzig · Ashburn, US
Harald F. Hess · Ravensburg, DE

Key dates

Filing date	Nov 21, 2007
Grant date	Aug 24, 2010
Priority date	—
Expiry date	May 24, 2028

Classification

Technology area (CPC G)Physics
CPC primaryG01N2021/6441
WIPO fieldMeasurement
WIPO sectorInstruments

Abstract

First activation radiation is provided to a sample that includes phototransformable optical labels (“PTOLs”) to activate a first subset of the PTOLs in the sample. First excitation radiation is provided to the first subset of PTOLs in the sample to excite at least some of the activated PTOLs, and radiation emitted from activated and excited PTOLs within the first subset of PTOLs is detecting with imaging optics. The first activation radiation is controlled such that the mean volume per activated PTOL in the first subset is greater than or approximately equal to a diffraction-limited resolution volume (“DLRV”) of the imaging optics.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.