Escherichia coli-derived vaccine and therapy against botulism

US7820411B2 · kind B2 · utility

Assignee

• Wisconsin Alumni Research Foundation · US

Inventors

• Michael Baldwin · Milwaukee, US
• Marite Bradshaw · Madison, US
• William H. Tepp · Stoughton, US
• Eric A. Johnson · Hudson, US
• Joseph T. Barbieri · Milwaukee, US
• Christina L. Pier · Fitchburg, US

Key dates

• Filing date: Feb 29, 2008
• Grant date: Oct 26, 2010
• Expiry date: Apr 14, 2028

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y02A50/30
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

A method of producing botulinum toxin C-terminal receptor binding domain (HCR) is disclosed. The one embodiment, the method comprises the steps of (a) preparing E. coli transformed with an expression vector comprising DNA encoding HCR protein, (b) inducing expression of the HCR protein at a reduced temperature in a culture media, and (c) purifying the HCR protein via extraction, wherein the extraction comprises a clarification by centrifugation and a filtration, wherein the purified HCR protein is at least 10 mg/L of culture medium.