Methods for producing soluble membrane-spanning proteins
US7829684B2 · kind B2 · utility
2Cited by
7References
58Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Aug 14, 2009 |
| Grant date | Nov 9, 2010 |
| Priority date | — |
| Expiry date | Aug 14, 2029 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2317/92
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods for producing membrane-spanning polypeptides in high yields, with native conformation, and/or in soluble form include solubilizing in non-ionic or zwitterionic detergents, as well as use of promoters and expression vectors for expressing high yields of membrane-spanning polypeptides in bacterial cells. Mutated promoters provide tight control of membrane-spanning polypeptides in bacterial cell hosts.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.