Modified polynucleotides for reducing off-target effects in RNA interference
US7834171B2 · kind B2 · utility
61Cited by
168References
9Claims
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Key dates
| Filing date | Sep 19, 2007 |
| Grant date | Nov 16, 2010 |
| Priority date | — |
| Expiry date | Jul 18, 2028 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2330/30
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods and compositions for performing RNA interference with decreased off-target effects are provided. The methods and compositions permit effective and efficient applications of RNA interference to applications such as diagnostics and therapeutics through the use of modifications to the siRNA. Uniquely modified siRNAs have been developed that reduce off-target effects incurred in gene-silencing. The modifications comprise 2′-O-alkyl or mismatch modification(s) at specific positions on the sense and/or antisense strands.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.