Methods for detection, identification and quantification of impurities
US7871519B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Mar 31, 2006 |
| Grant date | Jan 18, 2011 |
| Priority date | — |
| Expiry date | Jun 13, 2027 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2030/8827
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Provided is a method comprising introducing a sample comprising a plurality of oligonucleotides into an ion pair high performance liquid chromatography column having a buffered mobile phase and allowing at least a portion of the oligonucleotides to separate; allowing the oligonucleotides to elute from the column; and introducing the oligonucleotides into a mass spectrometer and quantifying at least a portion of the oligonucleotides by mass spectrometry. In the method, at least a portion of the oligonucleotides are co-eluting oligonucleotides that differ in mass by no more than 20%; and the buffered mobile phase causes at least 50 mole percent of co-eluting oligonucleotides to have the same charge when they enter the mass spectrometer.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.