DNA polymerases having improved labeled nucleotide incorporation properties

US7897738B2 · kind B2 · utility

Assignee

• Applied Biosystems, LLC · US

Inventors

• John Brandis · Austin, US
• Curtis Bloom · Chino Hills, US
• John Richards · Warrington, US

Key dates

• Filing date: Dec 9, 2005
• Grant date: Mar 1, 2011
• Expiry date: Jun 13, 2026

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6869
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present teachings relate to DNA polymerases that have an F667Y mutation as defined with respect to Taq DNA polymerase and exhibit reduced discrimination against labeled nucleotides into polynucleotides.The teachings also include kits comprising the subject DNA polymerases and numerous methods of using the subject DNA polymerases, including uses for chain termination sequencing and PCR.The present teachings relate to DNA polymerases that have an F667Y mutation as defined with respect to Taq DNA polymerase and exhibit reduced discrimination against labeled nucleotides into polynucleotides. The teachings also include kits comprising the subject DNA polymerases and numerous methods of using the subject DNA polymerases, including uses for chain termination sequencing and PCR.