Light emission modifiers and their uses in nucleic acid detection, amplification and analysis
US7919242B2 · kind B2 · utility
2Cited by
2References
25Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jun 23, 2006 |
| Grant date | Apr 5, 2011 |
| Priority date | — |
| Expiry date | Jan 18, 2027 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/975
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to methods and reagents for modifying the emission of light from labeled nucleic acids for the purpose of real time detection, analysis, and quantitation of nucleic acid sequences, e.g., using singly labeled probes. These methods and reagents exploit advantageous properties of thiazine dyes and diazine dyes. Furthermore, the use of these light emission modifiers in background reduction, nucleic acid duplex stabilization and other uses is also described. Related kits, reaction mixtures and integrated systems are described.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.