Methods for direct visualization of active synapses
US7923015B2 · kind B2 · utility
15Cited by
15References
23Claims
0Family size
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Key dates
| Filing date | Apr 6, 2004 |
| Grant date | Apr 12, 2011 |
| Priority date | — |
| Expiry date | Apr 6, 2024 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/00
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A method for visualizing an active synapse wherein said method comprises: (a) exposing cells forming the active synapse to a biomarker comprising at least fragment C of tetanus toxin and a reporter protein; and (b) visualizing the biomarker; wherein the accumulation of the biomarker into dendritic spines of the cells allows visualization of an active synapse. Also, a method for screening molecules capable of modulating synapse activity is provided. A kit useful for the early diagnosis of neurodegenerative disease comprises a biomarker comprising at least fragment C of tetanus toxin and a reporter protein.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.