High fidelity DNA polymerase compositions and uses therefor
US7932070B2 · kind B2 · utility
58Cited by
2References
50Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Aug 23, 2002 |
| Grant date | Apr 26, 2011 |
| Priority date | — |
| Expiry date | May 13, 2024 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N9/1252
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The subject invention relates to compositions comprising an enzyme mixture which comprises a first enzyme and a second enzyme, where the first enzyme comprises a DNA polymerization activity and the second enzyme comprises an 3′-5′ exonuclease activity and a reduced DNA polymerization activity. The invention also relates to the above compositions in kit format and methods for high fidelity DNA synthesis using the subject compositions of the invention.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.