Emulsion PCR and amplicon capture
US7947477B2 · kind B2 · utility
86Cited by
4References
32Claims
0Family size
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Key dates
| Filing date | Jan 25, 2010 |
| Grant date | May 24, 2011 |
| Priority date | — |
| Expiry date | Jan 25, 2030 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB01J2219/00648
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
Compositions and methods of use are disclosed for clonally amplifying target polynucleotide sequences in solution and attaching the amplicons to a surface by activation of a masked binding moiety. In an embodiment, the amplicons comprise the masked binding moiety and the surface comprises a binding partner of the binding moiety. Upon activation of the binding moiety, the amplicons bind to the binding partner on the surface. In a non-limiting example, the masked binding moiety is caged biotin or caged fluorescein, while the corresponding binding partner is avidin or an anti-fluorescein antibody.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.