Multiple RNA polymerase III promoter expression constructs

US7985581B2 · kind B2 · utility

Assignee

• Alnylam Pharmaceuticals, Inc. · US

Inventors

• Catherine Pachuk · Cambridge, US
• Daniel E. McCallus · Cambridge, US
• Chandrasekhar Satishchandran · Cambridge, US
• Martin D. Sigg, JR. · Philadelphia, US

Key dates

• Filing date: Aug 23, 2005
• Grant date: Jul 26, 2011
• Expiry date: Dec 27, 2026

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y02A50/30
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Expression constructs comprising at least two different RNA polymerase III promoters, wherein each promoter is operably linked to a nucleic acid sequence encoding an RNA effector molecule, are disclosed herein. Further provided are expression constructs comprising multiple polymerase III promoters operably linked to sequences encoding short hairpin RNA molecules, which may comprise single and/or multiple fingers. The provided constructs are useful for in vivo delivery of RNA molecules effective in gene silencing, including of viral genes including HBV and HCV.