Systems and methods for baseline correction using non-linear normalization
US8005628B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Jan 29, 2008 |
| Grant date | Aug 23, 2011 |
| Priority date | — |
| Expiry date | Apr 7, 2028 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2201/12746
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Systems and methods for normalizing detected emission data collected in real-time polymerase chain reaction (RT-PCR) and other reactions, are provided. In some embodiments, a sample plate can be loaded with a fluorescent dye and subjected to a real-time PCR reaction. During the initial cycles, detected emissions that correspond to the background signal contributed by the plate, buffer, and other non-reactant pieces of the reaction system and chemistry can be identified. The raw emission data can be normalized by dividing the emission data by the identified baseline signal. According to various embodiments, the normalized amplification profile can normalize to an initial value of 1, because the actual signal emerges from the baseline at the point exponential growth begins. A normalized amplification profile based on a ratio to the baseline can create a more uniformly scaled amplification curve across different samples, filters, wells, dyes, or machines.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.