Lipid-encapsulated polyanionic nucleic acid
US8021686B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Feb 8, 2008 |
| Grant date | Sep 20, 2011 |
| Priority date | — |
| Expiry date | Oct 30, 2029 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T428/2984
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
Methods for the preparation of a lipid-nucleic acid composition are provided. According to the methods, a mixture of lipids containing a protonatable or deprotonatable lipid, for example an amino lipid and a lipid such as a PEG- or Polyamide oligomer-modified lipid is combined with a buffered aqueous solution of a charged therapeutic agent, for example polyanionic nucleic acids, to produce particles in which the therapeutic agent is encapsulated in a lipid vesicle. Surface charges on the lipid particles are at least partially neutralized to provide surface-neutralized lipid-encapsulated compositions of the therapeutic agents. The method permits the preparation of compositions with high ratios of therapeutic agent to lipid and with encapsulation efficiencies in excess of 50%.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.