Compositions of orthogonal lysyl-tRNA and aminoacyl-tRNA synthetase pairs and uses thereof

US8030028B2 · kind B2 · utility

Assignee

• THE SCRIPPS RESEARCH INSTITUTE · US

Inventors

• J. Christopher Anderson · San Francisco, US
• Ning Wu · Boston, US
• Stephen Santoro · Daly City, US
• Peter G. Schultz · Village of La Jolla, US

Key dates

• Filing date: Nov 12, 2009
• Grant date: Oct 4, 2011
• Expiry date: Nov 12, 2029

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12P21/00
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Compositions and methods of producing components of protein biosynthetic machinery that include orthogonal lysyl-tRNAs, orthogonal lysyl-aminoacyl-tRNA synthetases, and orthogonal pairs of lysyl-tRNAs/synthetases, which incorporate homoglutamines into proteins are provided in response to a four base codon. Methods for identifying these orthogonal pairs are also provided along with methods of producing proteins with homoglutamines using these orthogonal pairs.