Modified amylases from Pseudomonas species

US8030050B2 · kind B2 · utility

Assignee

• DANISCO A/S · DK

Inventors

• Casper Tune Berg · Copenhagen, DK
• Patrick Derkx · Copenhagen, DK
• Carol Fioresi · Redwood City, US
• Gijsbert Gerritse · Heerjansdam, NL
• Anja Hemmingen Kellet-Smith · Tønder, DK
• Karsten Matthias Kragh · Aarhus, DK
• Wei Liu · Palo Alto, US
• Andrew Shaw · San Francisco, US
• Bo Spange Sørensen · Skanderborg, DK
• Charlotte Refdahl Thoudahl · Greve Strand, DK

Key dates

• Filing date: Sep 22, 2006
• Grant date: Oct 4, 2011
• Expiry date: Jan 16, 2028

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N9/2428
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The invention describes a PS4 variant polypeptide derivable from a parent polypeptide having amylase activity selected from the group consisting of: (a) a polypeptide comprising an amino acid mutation at each of positions 33, 34, 121, 134, 141, 146, 157, 161, 178, 179, 223, 229, 272, 303, 307, 309 and 334; (b) a polypeptide comprising an amino acid mutation at each of positions 33, 34, 121, 134, 141, 145, 146, 157, 178, 179, 223, 229, 272, 303, 307 and 334; (c) a polypeptide comprising an amino acid mutation at each of positions 33, 34, 121, 134, 141, 146, 157, 178, 179, 223, 229, 272, 303, 307, 309 and 334; and (d) a polypeptide comprising an amino acid mutation at each of positions 3, 33, 34, 70, 121, 134, 141, 146, 157, 178, 179, 223, 229, 272, 303, 307, 309 and 334; with reference to the position numbering of a Pseudomonas saccharophilia exoamylase sequence shown as SEQ ID NO: 1, uses of such a polypeptide as a food or feed additive, and nucleic acids encoding such.