Methods and compositions for purifying human factor VIII, factor VIII-like proteins or fragments thereof

US8058017B2 · kind B2 · utility

Assignee

• Dyax Corp. · US

Inventors

• Jinan Yu · Acton, US
• M. Daniel Potter · Acton, US
• Brian D. Kelley · Medford, US
• Jeffrey S. Deetz · Melrose, US
• James E. Booth · Andover, US

Key dates

• Filing date: Jan 22, 2010
• Grant date: Nov 15, 2011
• Expiry date: Jan 22, 2030

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K14/755
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Provided herein are methods and compositions for purifying human factor VIII, human factor VIII-like peptide or fragments thereof. The methods comprise immobilizing a binding molecule for human factor VIII, human factor VIII-like protein or fragments thereof to a solid support, contacting the immobilized binding molecule with a solution containing the human factor VIII, human factor VIII-like protein or fragments thereof, and purifying the factor by separating the solution from the solid support.