Methods of producing and sequencing modified polynucleotides
US8058030B2 · kind B2 · utility
77Cited by
18References
11Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Dec 7, 2009 |
| Grant date | Nov 15, 2011 |
| Priority date | — |
| Expiry date | Jan 1, 2030 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6869
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention encompasses methods for producing a modified polynucleotide sequence that comprises a (e.g., one or more) phosphorothiolate linkage, methods for determining a polynucleotide sequence comprising a (e.g., one or more) phosphorothiolate linkage, and methods for separating forward and reverse extension products that comprise a (e.g., one or more) phosphorothiolate linkage. The invention also encompasses kits for producing and/or determining the sequence of a modified polynucleotide that comprises a (e.g., one or more) phosphorothiolate linkage.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.