Methods of determining potency of chemically-synthesized oligonucleotides
US8093061B2 · kind B2 · utility
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Key dates
| Filing date | Oct 18, 2007 |
| Grant date | Jan 10, 2012 |
| Priority date | — |
| Expiry date | Oct 18, 2027 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/143333
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Provided herein are methods for determining potency of RNAi agents. Such methods include, but are not limited to, cell-based and cell-free assays that measure binding of an RNAi agent with Ago2 or that measure Ago2 activity in the presence of such RNAi agents. Also provided are assays that determine potency of RNAi agents by assessing their ability to compete with other RNAi agents, including control RNAi agents, for binding and/or activation of Ago2.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.