Engineered microorganisms capable of producing target compounds under anaerobic conditions
US8097440B1 · kind B1 · utility
Assignees
Inventors
Key dates
| Filing date | Oct 10, 2011 |
| Grant date | Jan 17, 2012 |
| Priority date | — |
| Expiry date | Oct 10, 2031 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY02E50/10
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention is generally provides recombinant microorganisms comprising engineered metabolic pathways capable of producing C3-C5 alcohols under aerobic and anaerobic conditions. The invention further provides ketol-acid reductoisomerase enzymes which have been mutated or modified to increase their NADH-dependent activity or to switch the cofactor preference from NADPH to NADH and are expressed in the modified microorganisms. In addition, the invention provides isobutyraldehyde dehydrogenase enzymes expressed in modified microorganisms. Also provided are methods of producing beneficial metabolites under aerobic and anaerobic conditions by contacting a suitable substrate with the modified microorganisms of the present invention.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.