Patent · US Active

Configuration of a laser scanning microscope for raster image correlation spectroscopy measurement and method for conducting and evaluating such a measurement

US8120771B2 · kind B2 · utility

1Cited by

2References

30Claims

0Family size

Assignee

Carl Zeiss Micromanaging GmbH · DE

Inventors

Stephan Wagner-Conrad · Jena, DE
Frank Hecht · Weimar, DE
Klaus Weisshart · Jena, DE
Ralf Netz · Jena, DE

Key dates

Filing date	Sep 30, 2008
Grant date	Feb 21, 2012
Priority date	—
Expiry date	Sep 30, 2028

Classification

Technology area (CPC G)Physics
CPC primaryG02B21/008
WIPO fieldOptics
WIPO sectorInstruments

Abstract

By means of an improved configuration method, mathematical transport models can be fitted to correlations determined by means of scanning fluorescence spectroscopy with few errors. With improved methods for carrying out or evaluating a raster image correlation spectroscopy measurement (RICS) measurement, the amount of data to be stored can be reduced and RICS correlations of high statistical quality can be determined within a short period of time. For a raster image correlation spectroscopy measurement, a best value for a sampling value is determined and is specified for a subsequent scanning process on a sample. In order to carry out or evaluate a RICS measurement, sampling values are acquired or a correlation is determined exclusively in a sample region within which a pixel time (ΔP) changes along a harmonically controlled scan axis (X) by less than, or at most by, a predetermined or predeterminable value.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.