Patent · US Expired

Method for making populations of defined nucleic acid molecules

US8133670B2 · kind B2 · utility

0Cited by
13References
26Claims
0Family size

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Key dates

Filing dateJun 2, 2004
Grant dateMar 13, 2012
Priority date
Expiry dateJun 30, 2024

Classification

  • Technology area (CPC Y)Emerging Cross-Sectional Technologies
  • CPC primaryY10S977/792
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

The present invention provides methods of making a population of nucleic acid molecules, wherein each nucleic acid molecule comprises a predetermined nucleic acid sequence, each of said methods comprising the steps of: (a) synthesizing, on a substrate, a population of nucleic acid molecules wherein: i) each synthesized nucleic acid molecule comprises a predetermined nucleic acid sequence; and ii) each synthesized nucleic acid molecule is localized to a defined area of said substrate; (b) harvesting said population of synthesized nucleic acid molecules from said substrate to yield harvested nucleic acid molecules; and (c) introducing said harvested nucleic acid molecules into vector molecules.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.