Patent · US Expired

System for multi color real time PCR

US8137616B2 · kind B2 · utility

14Cited by

16References

4Claims

0Family size

Assignee

Roche Molecular Systems, Inc. · US

Inventors

Gregor Sagner · Penzberg, DE
Ingrid Bechler · Gelting, DE
Jochen Bolte · Wiesbaden, DE
Dieter Heindl · Munich, DE
Hans-Peter Josel · Weilheim, DE
Martin Gutekunst · Eberfing, DE
Rudolf Sebl · Penzberg, DE
Christoph Mueller · Bern, CH

Key dates

Filing date	Apr 1, 2004
Grant date	Mar 20, 2012
Priority date	—
Expiry date	Apr 1, 2024

Classification

Technology area (CPC G)Physics
CPC primaryG01N2021/6421
WIPO fieldMeasurement
WIPO sectorInstruments

Abstract

The invention is directed to a system for performing multi-color real time PCT, comprising a flexible real time PCT instrument and a specific composition or reaction mixture for performing multiplex PCR: In particular, the present invention is directed to a composition or reaction mixture which comprises at least 3, preferably 4-5 and most preferably exactly 4 pairs of FRET hybridization probes. Each pair of said hybridization probes consists of a FRET donor probe carrying a FRET donor moiety and a FRET acceptor probe carrying a FRET acceptor moiety having an emission maximum between 550 and 710 nm.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.