Modified amylases from pseudomonas species, methods of making and uses thereof

US8137944B2 · kind B2 · utility

Assignee

• DANISCO A/S · DK

Inventors

• Casper Tune Berg · Copenhagen, DK
• Patrick Derkx · Copenhagen, DK
• Carol Fioresi · Redwood City, US
• Gijsbert Gerritse · Heerjansdam, NL
• Anja Hemmingen Kellet-Smith · Tønder, DK
• Karsten Matthias Kragh · Aarhus, DK
• Wei Liu · Palo Alto, US
• Andrew Shaw · San Francisco, US
• Bo Spange Sørensen · Skanderborg, DK
• Charlotte Refdahl Thoudahl · Greve Strand, DK

Key dates

• Filing date: Jul 7, 2005
• Grant date: Mar 20, 2012
• Expiry date: Dec 15, 2026

Classification

• Technology area (CPC A): Human Necessities
• CPC primary: A23V2002/00
• WIPO field: Food chemistry
• WIPO sector: Chemistry

Abstract

Variant polypeptides derivable from a parent polypeptide having non-maltogenic exoamylase activity, in which the variant polypeptides comprise an amino acid mutation at one or more positions selected from the group consisting of: 121, 161, 223, 146, 157, 158, 198, 229, 303, 306, 309, 316, 353, 26, 70, 145, 188, 272, 339, with reference to the position numbering of a Pseudomonas saccharophila exoamylase sequence shown as SEQ ID NO: 1.