Production of an α-carboxyl-ω-hydroxy fatty acid using a genetically modified Candida strain
US8158391B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | May 6, 2009 |
| Grant date | Apr 17, 2012 |
| Priority date | — |
| Expiry date | Dec 20, 2029 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12P7/44
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A substantially pure Candida host cell for the production of a α-carboxyl-ω-hydroxy fatty acid having a carbon chain length in the range from C6 to C22, a α,ω-dicarboxylic fatty acid having a carbon chain length in the range from C6 to C22, or mixtures thereof, is provided. The Candida host cell is characterized by a first genetic modification class and a second genetic modification class. The first genetic modification class comprises one or more genetic modifications that disrupt the peroxisomal β-oxidation pathway. The second genetic modification class comprises one or more genetic modifications that collectively or individually disrupt at least one gene selected from the group consisting of a CYP52A type cytochrome P450, a fatty alcohol oxidase, and an alcohol dehydrogenase.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.