Light emission modifiers and their uses in nucleic acid detection, amplification and analysis
US8163484B2 · kind B2 · utility
2Cited by
2References
15Claims
0Family size
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Key dates
| Filing date | Sep 1, 2009 |
| Grant date | Apr 24, 2012 |
| Priority date | — |
| Expiry date | May 19, 2030 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/975
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to methods and reagents for modifying the emission of light from labeled nucleic acids for the purpose of real time detection, analysis, and quantitation of nucleic acid sequences, e.g., using singly labeled probes. These methods and reagents exploit advantageous properties of thiazine dyes and diazine dyes. Furthermore, the use of these light emission modifiers in background reduction, nucleic acid duplex stabilization and other uses is also described. Related kits, reaction mixtures and integrated systems are described.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.