Method for direct amplification from crude nucleic acid samples
US8173401B2 · kind B2 · utility
42Cited by
7References
11Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jun 30, 2009 |
| Grant date | May 8, 2012 |
| Priority date | — |
| Expiry date | Jun 30, 2029 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/686
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present teachings relate to improved methods, kits, and reaction mixtures for amplifying nucleic acids. In some embodiments a novel direct buffer formulation is provided which allows for the direct amplification of the nucleic acids in a crude sample with minimal sample purification.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.