Methods and composition for the production of orthogonal tRNA-aminoacyltRNA synthetase pairs

US8183012B2 · kind B2 · utility

Assignees

• THE SCRIPPS RESEARCH INSTITUTE · US
• The Regents of the University of California · US

Inventors

• Peter G. Schultz · Village of La Jolla, US
• Lei Wang · Beijing, CN
• John Christopher Anderson · Kihei, US
• Jason W. Chin · Pidley, GB
• David R. Liu · Lexington, US
• Thomas J. Magliery · Columbus, US
• Eric Meggers · Philadelphia, US
• Ryan A. Mehl · San Diego, US
• Miro Pastrnak · San Diego, US
• Steven William Santoro · Cambridge, US
• Zhiwen Zhang · Austin, US

Key dates

• Filing date: Dec 10, 2004
• Grant date: May 22, 2012
• Expiry date: Apr 29, 2026

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12P21/02
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

This invention provides compositions and methods for generating components of protein biosynthetic machinery including orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, and orthogonal pairs of tRNAs/synthetases. Methods for identifying orthogonal pairs are also provided. These components can be used to incorporate unnatural amino acids into proteins in vivo.