Patent · US Active

Method to quantify siRNAs, miRNAs and polymorphic miRNAs

US8187815B2 · kind B2 · utility

2Cited by

6References

21Claims

0Family size

Assignee

Applied Biosystems, LLC · US

Inventors

Ruoying Tan · Palo Alto, US
Caifu Chen · Brookline, US
Karl Guegler · Menlo Park, US

Key dates

Filing date	Jan 27, 2011
Grant date	May 29, 2012
Priority date	—
Expiry date	Jan 27, 2031

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12Q1/6851
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

The present teachings provide methods, compositions, and kits for quantifying target polynucleotides. In some embodiments, a reverse stem-loop ligation probe is ligated to the 3′ end of a target polynucleotide, using a ligase that can ligate the 3′ end of RNA to the 5′ end of DNA using a DNA template, such as T4 DNA ligase. Following digestion to form an elongated target polynucleotide with a liberated end, a reverse transcription reaction can be performed, followed by a PCR. In some embodiments, the methods of the present teachings can discriminate between polymorphic polynucleotides that vary by as little as one nucleotide.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.