Method for characterizing reversible association of macromolecules at high concentration
US8195405B2 · kind B2 · utility
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30Claims
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Key dates
| Filing date | Jun 30, 2009 |
| Grant date | Jun 5, 2012 |
| Priority date | — |
| Expiry date | Sep 8, 2030 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N21/53
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A new method is presented for characterizing the associative properties of a solution of macromolecules at high concentration. Sample aliquots spanning a range of concentrations are injected sequentially into a light scattering photometer. Equilibrium association constants and association stoichiometry are derived from an analysis of the angular and concentration dependence of the scattering signals. Thermodynamic nonideality, which becomes important at high concentrations, is dealt with in the analysis in a simplified manner which is applicable to multiple associated species.
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