Synthetic expression vectors for insect cells
US8198079B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Apr 28, 2008 |
| Grant date | Jun 12, 2012 |
| Priority date | — |
| Expiry date | Feb 2, 2029 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2830/75
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention is directed at optimized expression vectors for the expression of native-like heterologous proteins in insect cells. Compositions of the invention are nucleotide sequences representing elements of an expression vector that when combined results in enhanced expression and secretion of heterologous proteins. The elements include sequences that define transcriptional activators, core promoters, secretion signals, and 3′ untranslated regions that are functional in insect cells. The elements contained in the optimized vectors are all synthetically derived or are modified variants of naturally occurring insect sequences. The expression vectors are useful for the expression of native-like proteins when protein encoding nucleotide sequences are operatively linked to the vectors. These vectors can be used to transform insect cells, which can then be cultured to produce the desired protein product. The expressed native-like proteins can be used in diagnostic, vaccine or other applications requiring large amounts of high quality proteins.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.