Three part assay for kinase or phosphatase activity
US8206941B2 · kind B2 · utility
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2References
6Claims
0Family size
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Key dates
| Filing date | Jun 16, 2009 |
| Grant date | Jun 26, 2012 |
| Priority date | — |
| Expiry date | Jul 6, 2030 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/542
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention relates to a method of determining kinase or phosphatase activity based on a three parts system. The method comprises contacting a binding partner which can bind phosphorylated peptides, a detection molecule and a substrate peptide. Determination of activities is achieved by measuring energy transfer between an energy donor and an energy acceptor that are present on the detection molecule and the substrate molecule.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.