Microorganism that displays biotin on cell surface
US8252577B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Apr 28, 2009 |
| Grant date | Aug 28, 2012 |
| Priority date | — |
| Expiry date | Aug 8, 2029 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N9/93
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Provided is a microorganism that can display, on the cell surface, any molecules other than a molecule comprising amino acids, more specifically, a microorganism that displays biotin on a cell surface. The microorganism is capable of co-expressing a biotinylating enzyme and an acceptor peptide having a sequence recognized by the biotinylating enzyme, wherein the acceptor peptide is expressed on the cell surface, so that lysine of the acceptor peptide is biotinylated to display biotin on the cell surface. Also provided is a method for displaying an intended molecule, including not only a molecule comprising amino acids but also any molecules, on a cell surface of a microorganism.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.