Method for detecting the presence of a single target nucleic acid in a sample
US8278071B2 · kind B2 · utility
223Cited by
123References
6Claims
0Family size
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Key dates
| Filing date | Aug 13, 2007 |
| Grant date | Oct 2, 2012 |
| Priority date | — |
| Expiry date | Feb 9, 2028 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/2575
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
A method comprising subjecting one or more sample portion(s) to a single amplification step, thereby amplifying a single molecule in the sample portion to a detectable level, and, in some embodiments, then determining whether the sample portion contains at least one molecule of the target nucleic acid. In some embodiments, the sample portion is in a porous sample structure, or in a sample chamber which comprises means for minimizing diffusion of the sample portion, or in a sample chamber which is inside a microcapillary device, or in a sample retaining means.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.