DNA polymerase compositions for quantitative PCR and methods thereof
US8283148B2 · kind B2 · utility
62Cited by
4References
25Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Dec 12, 2003 |
| Grant date | Oct 9, 2012 |
| Priority date | — |
| Expiry date | Jun 24, 2029 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6869
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention relates to the generation and characterization of Archaeal DNA polymerase mutants with deficient 3′-5′ exonuclease activity and reduced base analog detection activity. The invention further provides for Archaeal DNA polymerase mutants with deficient 3′-5′ exonuclease activity and reduced base analog detection activity containing additional mutations that modulate other DNA polymerase activities including DNA polymerization or reverse transcriptase activity. The invention also discloses methods and applications of DNA polymerases with deficient 3′-5′ exonuclease activity and reduced base analog detection activity.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.