Generic kinase/phosphatase assay with single readout
US8309310B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Sep 1, 2008 |
| Grant date | Nov 13, 2012 |
| Priority date | — |
| Expiry date | Jan 30, 2029 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/485
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention relates to a generic method for detecting a kinase or phosphatase activity. The method comprises the following steps: incubating a kinase or phosphatase activity sample with a kinase or phosphatase substrate molecule comprising either a fluorophore having a detectable readout or molecule with an aromatic group which serves as a quencher of the fluorophore, incubating the mixture of step a) with a detection entity comprising either a fluorophore or a molecule with an aromatic group and a binding partner, wherein the substrate molecule and the detection entity are capable of binding to the binding partner and the binding of the substrate molecule and the detection entity to the binding partner lead to an altered readout of the fluorophore, and measuring the readout of the fluorophore in the mixture of step b), wherein an altered readout of the fluorophore compared to a blank is indicative for the presence of a kinase or phosphatase activity in the sample.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.