Regioselective alkane hydroxylation with a mutant AlkB enzyme
US8309333B1 · kind B1 · utility
2Cited by
2References
7Claims
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Key dates
| Filing date | Nov 16, 2009 |
| Grant date | Nov 13, 2012 |
| Priority date | — |
| Expiry date | Nov 6, 2030 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY02E50/10
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
AlkB from Pseudomonas putida was engineered using in-vivo directed evolution to hydroxylate small chain alkanes. Mutant AlkB-BMO1 hydroxylates propane and butane at the terminal carbon at a rate greater than the wild-type to form 1-propanol and 1-butanol, respectively. Mutant AlkB-BMO2 similarly hydroxylates propane and butane at the terminal carbon at a rate greater than the wild-type to form 1-propanol and 1-butanol, respectively. These biocatalysts are highly active for small chain alkane substrates and their regioselectivity is retained in whole-cell biotransformations.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.