Regioselective alkane hydroxylation with a mutant CYP153A6 enzyme
US8361769B1 · kind B1 · utility
30Cited by
2References
11Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Nov 16, 2009 |
| Grant date | Jan 29, 2013 |
| Priority date | — |
| Expiry date | Mar 29, 2031 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY02E50/10
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Cytochrome P450 CYP153A6 from Myobacterium sp. strain HXN1500 was engineered using in-vivo directed evolution to hydroxylate small-chain alkanes regioselectively. Mutant CYP153A6-BMO1 selectively hydroxylates butane and pentane at the terminal carbon to form 1-butanol and 1-pentanol, respectively, at rates greater than wild-type CYP153A6 enzymes. This biocatalyst is highly active for small-chain alkane substrates and the regioselectivity is retained in whole-cell biotransformations.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.