Total internal reflection microscope apparatus and method for analyzing fluorescent sample
US8362449B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Nov 30, 2009 |
| Grant date | Jan 29, 2013 |
| Priority date | — |
| Expiry date | Nov 30, 2029 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2021/6432
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
An object of the present invention relates to observation of single molecule fluorescence while temperature of a sample solution is controlled by a temperature controller and intrinsic fluorescence of the temperature controller is avoided, in a total internal reflection microscope. The present invention relates to provision of an opening at areas of the temperature controller through which incident light and reflected light pass, and configuration adopting a material with intrinsic fluorescence lower than that of the other parts, in a total internal reflection microscope including a prism and the temperature controller. The present invention enables intrinsic fluorescence of the temperature controller to be suppressed, which allows highly sensitive fluorescence observation while controlling sample solution temperature with high precision. For instance, this in turn allows the throughput of single molecule DNA sequencing using a total internal reflection microscope to be improved.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.